The teams have been divided into 10 zones but the league will be suspended after the first three matches to make way for the FA Cup knockout qualifications.
All the teams participating in the regional second division league would be regrouped to play in the knockout fixtures to battle for six of the places for next season's FA Cup spots.
The Eastern Regional Division Two League would be played over two rounds in each zone and the top two teams from each zone qualifies to play in the middle league.
The best four teams from the middle league will qualify to play in a super middle league.
The winner of the super middle league then qualifies to represent the region in the National Division One league.